Thus, novel target genes for the improvement of lysine production could be identified in a discovery-driven approach based on global gene expression analysis. Journal of bacteriology 187, 6005—6018 2005. A cis-dominant mutation linked to argF, the structural gene specifying ornithine carbamoyltransferase, and affecting the control of the synthesis of this enzyme has been obtained. Current amino acid production processes with C. Applied and environmental microbiology 73, 2079—2084 2007. Biotechnology journal 10, 1170—1184 2015.
Furthermore, batch cultivation of the engineered C. In addition, Crh and the similar phosphoenolpyruvate:sugar phosphotransferase protein Hpr phosphorylate and activate glycerol kinase ,. Electronic supplementary material The online version of this article doi:10. Engineering Corynebacterium glutamicum for the production of pyruvate. The protein is organized into four regions, three of which are functional domains as indicated previously by genetic analysis. Applied microbiology and biotechnology 77, 1053—1062 2008. Topics discussed are the amino acid biosynthetic pathways, their genetic and biochemical regulation, transport of amino acids and genomics of producing microorganisms.
Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum. Hielscher GmbH, Teltow, Germany at an amplitude of 60% and a duty cycle of 0. Carbamate kinase had 37% sequence similarity and 13. Biocatalytic imine reduction and reductive amination of ketones. Lysine production by recombinant C. Amino Acids Biosynthesis presents the current knowledge of fundamental as well as applied microbiology of amino acids. Box 100131 33501 Bielefeld Germany Room W6-134 phone: +49-521-106 5611 fax: +49-521-106 5626 Consultation hours: Wednesday 8.
Coverage details the amino acid biosynthetic pathways, their genetic and biochemical regulation, transport of amino acids and genomics of producing microorganisms. Thus, the arcA and arcC genes do not appear to be closely related to arginine biosynthetic genes, whereas it had previously been shown that the arcB gene has a high degree of identity with the arginine biosynthetic argF genes of P. Thus l-arginine production could reach 45. Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: growth and lysine production. The first feeding phase with 26. The product N-acetylcitrulline is deacetylated by an enzyme that is also involved in the provision of ornithine from acetylornithine; this is an important metabolic function, as ornithine itself can become essential as a source of other metabolites.
This interpretation has been given previously for similar findings in other metabolic pathways, notably for the control of the enzymes of lactose fermentation and of histidine and tryptophan biosynthesis. Comparison of the proteolytic susceptibilities of homologous l-amino acid, d-amino acid, and N-substituted glycine peptide and peptoid oligomers. In rifampin-free conditions, rpoC mutation was associated with M. Therefore, the pyruvate overproducing C. In this work, the amino acids and B vitamins essential for sustaining the growth of C. Syntheses of N-methylated amino acids by chemical and biocatalytic approaches are known, but often show incomplete stereoselectivity, low yields or expensive co-factor regeneration. The great majority of Pyrococcus proteins are typical archaeal proteins and their phylogenetic pattern agrees with its position near the root of the archaeal tree.
The two acetylating activities appear to be properties of one enzyme with one portion more easily denatured. The hexameric regulatory protein ArgR formed by arginine-mediated dimerization of identical trimers governs the expression of genes required for arginine metabolism and some other genes in mesophilic and moderately thermophilic bacteria. Upon expression of the α-amylase gene amyA from Streptomyces griseus, C. Only recently, researchers have investigated the potential of amino acid-producing strains of Corynebacterium glutamicum and Escherichia coli for polyamine production. Four hours after induction, cell samples were harvested and treated as described in reference. Metabolic pathways could be reconstructed for all amino acids and B vitamins with the exception of biotin vitamin B7 and folate vitamin B9. .
Analysis of the argJ sequence revealed that the argJ coded a polypeptide of 388 amino acids with a calculated molecular weight of 39. The transcription of both genes is not affected by the presence of arginine in the growth medium. The plasmid identified, which allowed functional complementation, contained part of gene cg3035, which contains an acetyltransferase domain in its amino acid sequence. A vast number of prokaryotic and eukaryotic microorganisms can synthesize arginine de novo from glutamate. Repression of enzyme synthesis by arginine was observed for most of the enzymes tested in T. For all experiments above 240 °C at all residence times, the yield of soluble peptides was in the range of 57-67% of the algae protein, whereas the maximum arginine yield 81.
Moreover, the promoter Parg would be used as a valid promoter to express objective genes for metabolic engineering in Corynebacteria. Glycerol utilization by Corynebacterium species is not well studied. Detection of the fluorescent derivatives was carried out with a fluorescence detector with an excitation wavelength of 230 nm and an emission wavelength of 450 nm. The high genomic G+C group of Actinobacteria possesses a variety of physiological and metabolic properties, and exhibits diverse lifestyles and ecological distribution. In Listeria, efficient utilization of glycerol and expression of glycerol utilization genes depend on the alternative sigma factor σ B.
Pyruvate:quinone oxidoreductase in Corynebacterium glutamicum: molecular analysis of the pqo gene, significance of the enzyme, and phylogenetic aspects. Selenomethionine-substituted recombinant protein was produced and selenomethionine substitution was verified by mass spectroscopy. The L-arginine productivity was 0. Higher growth level and enhanced glucose consumptions were also observed in C. Growth of the empty vector control and of the strain expressing only the glycerol facilitator gene glpF was not perturbed on glucose-glycerol medium Fig.
Applied and environmental microbiology 80, 3541—3550 2014. This site is preceded by a typical B. Possible effects due to substrate or product toxicity were detected in growth experiments with C. The constructed plasmid was used to transform C. The Km values were 0. N-methylation of peptides and proteins: an important element for modulating biological functions. To avoid foaming the antifoam Struktol® J647 was added manually when necessary.